Figure 2.
DSF/Cu kills thyroid cancer cells in an ROS-dependent way. (A) BRAFV600E-mutated thyroid cancer cell lines 8305C, 8505C and IHH4 were treated with 0.5 μM DSF/Cu alone or in combination with 2 mM NAC for 48 h, followed by a 1.5 h incubation with ROS-sensitive fluorescent dye DCF-DA. The ROS-positive cells were measured by flow cytometer (upper panels), and the mean fluorescence intensity of three independent experiments was then calculated by Student’s t test (lower panels). (B) The indicated cells were treated with 80 nM DSF/Cu alone or in combination with 2 mM NAC for 48 h, and cell viability was then measured by MTT assay. (C) Colony formation assay was performed when these cells were treated with DSF/Cu (8305C, 8505C and BCPAP: 50 nM, IHH4: 40 nM) alone or in combination with 2 mM NAC for 8–10 days. Data presented as means ± SD. ** p < 0.01; *** p < 0.001.