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. 2023 Feb 8;24(4):3418. doi: 10.3390/ijms24043418

Figure 5.

Figure 5

DSF/Cu relieves the rebound of MAPK/ERK and the activation of PI3K/AKT pathways caused by PLX4032. (A) The indicated cells were treated with 4 μM PLX4032 for 0 h, 6 h, 12 h, 24 h and 48 h, and Western blot analysis was then used to detect the levels of phosphorylated HER3 (p-HER3), total HER3 (t-HER3), p-AKTS473, p-AKTT308, t-AKT, p-ERK and t-ERK. (B) These cells were treated with the combination of 4 μM PLX4032 and 0.5 μM DSF/Cu, and Western blot analysis was similarly performed to detect the levels of the above molecules. (C) The same cell lines were treated with 0.5 μM DSF/Cu for 0 h, 12 h, 24 h and 48 h, and Western blot analysis was used to determine the levels of p-HER3 and t-HER3. (D) Western blot analysis of p-HER3 and t-HER3 in the indicated cells treated with 0.5 μM DSF/Cu alone or in combination with 2 mM NAC. β-Actin was used as a loading control.