Table 1.
Characteristics of 55 polymorphic microsatellite markers developed in this study for population genetic diversity analysis of Bipolaris sorokiniana isolates.
| Locus | Forward Primer Sequence (5′-3′) | Reverse Primer Sequence (5′-3′) | Tm (°C) | Allele Size (bp) | Motif | Repeat |
|---|---|---|---|---|---|---|
| BS-1 | GGAGATGTGTGTCATGGTGC | GGTCTTGATATTCCGTGTGCG | 58 | 265 | AGT | 44 |
| BS-2 | ATGTCACTTCCGACTCCAGC | TTTTGCTTCGGTTGCTTCGG | 59 | 224 | ACA | 42 |
| BS-3 | TGAGATGGTTGCAAAGGGGG | TCAACTCCATATTGCTTGGACC | 60 | 243 | AGA | 34 |
| BS-4 | GTTCTTGTTCTGCAGGTGCG | GAACAGCAGAGAAAAGGCGC | 60 | 214 | ACT | 30 |
| BS-5 | CACAGATGCTTTATCGCGCG | GCAACTGAAAACGGCAAATCC | 60 | 183 | GTA | 24 |
| BS-6 | GATTTTGATCGAAGGGGCGG | ACCTCATATGCGCACAAAAGG | 59 | 157 | TTC | 24 |
| BS-7 | TGGATTTGTCGGAGTTGAATTGC | TTTCCAACGGAAATTCGCGG | 60 | 199 | CTT | 21 |
| BS-8 | TGAGGATGAGGTTGTTGCGG | AACATACGCCCACCTCATCC | 60 | 174 | GAG | 21 |
| BS-9 | CGGTTAGCCACAGCAAAGC | TGTATTGTTCAAGCTGGCGC | 59 | 153 | TAG | 20 |
| BS-10 | CAACATGCTCGTTACCGTGG | TCACGCATCTAAGCAGCAGC | 59 | 125 | TAC | 20 |
| BS-11 | GGAACCTACTCCGACGTTGC | ATGTACAGACGCACGTCAGC | 60 | 209 | TGA | 18 |
| BS-12 | ACGGGTAAATCATCGGTGCC | TGGTGCAGGTATGAAGACGG | 60 | 175 | ACT | 18 |
| BS-13 | TTGCTGCTGCCTTGTATTGC | GCGTGCTGCAACAATGGG | 59 | 130 | ACT | 18 |
| BS-14 | ACGAGTCCTTTTTACCACAGC | ATCTGGCGTACTTTCCGTCC | 58 | 177 | GAA | 17 |
| BS-15 | GACACACTCGACTCGATGCC | CGCGAGGTTACTGGGATTGG | 60 | 135 | ACA | 17 |
| BS-16 | AGATTATCAGGCCTCCACAGG | CTCTCCAGGCACCAACCG | 58 | 191 | ATC | 16 |
| BS-17 | ACACTCGCCTTTAGTTTGGC | TGGTATGTCGTCCCAAAGCC | 58 | 180 | TTC | 16 |
| BS-18 | TCCACCCCAATTCTATACTTACTACC | TAATCAGAGGGGCAAAGGGC | 59 | 209 | TAG | 15 |
| BS-19 | ACCGTCCTACCCAGATACCC | TTGAGGATGGGGTGGGATCC | 60 | 184 | AGA | 15 |
| BS-20 | TTGCCCATTGCTCGTTACCC | GAGGGGTTTCAGCAGTAGGG | 60 | 183 | CTA | 14 |
| BS-21 | AGGCTGAAGCTGACAAAGGC | TTGGAGGAGAAGGAGGACGG | 60 | 182 | GAA | 14 |
| BS-22 | CGAGCACACAGTCGTCTAGG | TTGTTCGTTTTGCGTGTCGG | 60 | 182 | TCA | 14 |
| BS-23 | AGGCATTCAGTCCGTTAGTCC | CTTATTGCCGGCTGCTTACG | 59 | 125 | GTCA | 16 |
| BS-24 | ATGTGGGAATACGGGGAAGG | TTCAGCCAAGTCTCTTGTGC | 59 | 205 | AGAA | 14 |
| BS-25 | AGACCATCTGTTGCCCAACC | CAGACTGATTCCTTGTCGAGC | 60 | 165 | ATAC | 14 |
| BS-26 | GCGTTTGCTTTCGATCGTCC | AGGCTGGAGAGGAGAGTTGG | 60 | 157 | AAGA | 12 |
| BS-27 | AGACATTGAGGCAGTCGTGG | GGAAAACAGGCCGTTGTTGC | 60 | 148 | GGCT | 12 |
| BS-28 | GACATCGTATCTGCCGTGGG | AAAGCTGTCAAATTGCGGGC | 60 | 174 | TACA | 11 |
| BS-29 | TCAAATGCAATGTATTCTCTACCCG | CACGTCCCATAACGGATTGC | 59 | 159 | TCTT | 11 |
| BS-30 | ACAACCTGCCACTATCACGG | CCTAGTGGATGGGCAATGGG | 60 | 169 | CCAT | 10 |
| BS-31 | TGCATCACTGTAAGCCCTGG | TCCCAGCTTCAATGCCTTGG | 60 | 167 | CATA | 10 |
| BS-32 | TTTTCTTTCTCTCCGCACGC | GTCTTGGGGGTGGACAAGG | 59 | 168 | AGAA | 9 |
| BS-33 | TTTTGATCGAGGTCTAACAGGC | GCTCAATCGAGGAACTATGCC | 58 | 168 | CTTT | 9 |
| BS-34 | GAATAGGGAGTGGACGAGAGC | ACAAACGCTGCGTAGATTTCC | 59 | 196 | AAAG | 8 |
| BS-35 | GATTGGGCCAGTTGAAACCG | TGCCACCCTCCTCTACTACC | 59 | 188 | GGTT | 8 |
| BS-36 | CTGGTAGCGGTAGTGGTAGC | CTTGTAGAGAGGAGCCCTGC | 59 | 158 | GTGTA | 16 |
| BS-37 | CGTTCATTTTCTCCGCCAGG | TGGCATATGAATCCTGGGGG | 59 | 165 | AAAAG | 12 |
| BS-38 | CATCAGCCAAACCGTTGACG | TGTACTCTACACGGATGCATACG | 60 | 176 | ACTAC | 9 |
| BS-39 | GCCCCTAGATGAGAACTCGC | GCGAAATTTGCTGCAATCCC | 59 | 178 | TCGTG | 8 |
| BS-40 | TCAGTATCTAGTGCGCACCC | TGTGCATTGTTGTGCTGTCC | 59 | 171 | TGCCC | 8 |
| BS-41 | CTTCCATACTAGTCGGTCCCC | GGCAGGGCTTTCTTTTTGGG | 59 | 178 | CACTA | 7 |
| BS-42 | GACTAGTACTGGGCGATGGC | ACCAATCCTACTCGGCATGC | 59 | 204 | CGCAA | 6 |
| BS-43 | CTGCCCTAGAGTAAGACGGC | TGGAGTGTGTTGCTGCTAGG | 59 | 166 | CATCC | 6 |
| BS-44 | CCTACCTCCTCCCACTACCC | AAGTGATAGTGCGGGTGTGG | 60 | 160 | CCATC | 6 |
| BS-45 | CCGTTCACATGCCGTAAACG | CTGGGCGTGGTGTTTGTCG | 60 | 151 | CCATC | 6 |
| BS-46 | CTTTGCATGTTCCTGACCCG | CTTGCAACTCCAACATGGCC | 59 | 259 | AGCAGT | 17 |
| BS-47 | TCGTCTACGCCACGAATAGC | CCTCTAATGCGACGCGACG | 59 | 249 | TAGATG | 11 |
| BS-48 | TCTAGGCGTAGAGTGCTCCC | CCTGTCGAGCTGAAAATGCG | 60 | 159 | GCTCAT | 10 |
| BS-49 | TTTCGCTGAAACTTGCTCGC | TGAAGCCGAAGATGAGCAGG | 60 | 121 | TCCTGT | 8 |
| BS-50 | GTATGGGGCAGACTGGTAGC | CTCGTCCACGTCTACATCCC | 59 | 199 | TGCTGT | 7 |
| BS-51 | CAAATGTCCGGCGATGTTGG | GCTAACATGCAGCCAACACC | 60 | 192 | TGATTC | 7 |
| BS-52 | TAGGACTTGTTGCGGCTAGG | ACATGCTACACGGACACACC | 59 | 179 | ACCAGC | 6 |
| BS-53 | TCCTTGTCCTTGTCCTTGTCC | AGCCCTATGGTCACGAATGC | 59 | 179 | TTCTCT | 6 |
| BS-54 | GGGCTGGACGAGTGATATGG | TGCTGATACCGTTGCTGTCG | 59 | 160 | AGGAGC | 6 |
| BS-55 | ATCTTTTCGTGCAGGGGAGG | TCGATCCTCAAATAGCGCCC | 60 | 158 | TGTGGC | 6 |