Figure 6.

Ruxolitinib decreased the differentiation of MDSCs to mature cells via STAT5 inhibition in vitro. BM‐derived MDSCs in vitro were cultured with GM‐CSF with or without different concentrations of ruxolitinib (0.1 μm, 1 μm, 10 μm) for 5 days. (a) Representative plots of macrophages (CD11b⁺F4/80⁺) and dendritic cells (CD11b⁺CD11c⁺) were shown. The percentages of differentiated mature cells (b) and the expression of costimulatory molecules CD80⁺ gated on CD11b⁺ cells (c) were indicated (n = 3). (d) The phosphorylation of STAT5 was examined by phosflow techniques (n = 3). (e) The levels of transcriptional factors related to MDSCs differentiation were monitored by real‐time PCR (n = 3). Data are expressed as mean ± standard error (SE). ***P < 0.001, ****P < 0.0001. These results are representative of three independent experiments.