TABLE 1.

Bacterial strains and plasmids used in this study

Designation	Relevant characteristics	Reference or source
S. enterica serovar Typhimurium strains
NCTC 12023	Wild type, identical to ATCC 14028	ATCCa
P10E11	sscA::mTn5	29
P9B7	ssaT::mTn5	29
HH102	sseB::aphT, Kmr, nonpolar mutation	11
HH103	HH102 containing psseB	11
HH104	sseC::aphT, Kmr, nonpolar mutation	11
HH109	ssaV::aphT, Kmr, nonpolar mutation	4
MvP101	sseD::aphT, Kmr, nonpolar mutation	20
MvP131	ssaB::luc lacZ	3
E. coli strain
M15	pREP4	Qiagen
Plasmids
pEM25	pMJH expressing SspH1-CyaA	24
pEM30	pMJH expressing SspH2-CyaA	24
pQE	His tag expression vectors, Ampr	Qiagen
pJD14	Codons 180 to 484 of sseC in pQE40	This study
pJD15	Codons 2 to 195 of sseD in pQE30	This study
psseB	sseB in pACYC184, Cmr	11

^aATCC, American Type Culture Collection.