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. 2023 Feb 25;14:1098. doi: 10.1038/s41467-023-36681-z

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Illustration depicting the strategy to specifically disrupt FSH receptor expression in the pituitary via stereotaxic delivery of an adeno-associated virus (AAV) encoding Cas9 and a guide RNA targeting Fshr into the pituitary. b Percentage of unmodified and modified alleles and c distribution of identified alleles around the predicted cleavage site determined by CRISPResso2 of the pituitary DNA from an AAV5-saCas9-Fshr injected mouse. d Representative images (scale bars = 50 μm) and e quantification of liver oil red O staining from AAV-Cas9 (with (n = 3 mice) or without (n = 5 mice) guide RNA against follicle-stimulating hormone receptor; Fshr) pituitary-injected mice. f Liver oil red O staining quantification in AAV-Cas9 (with guide RNA against follicle-stimulating hormone receptor; Fshr) pituitary-injected adrenalectomized or sham-operated female mice. g Model of gonadotrope actions on the gonads and the pituitary (filled blue arrow illustrates gonadotrope actions on the gonads; dashed blue arrows indicate indirect gonadotrope effects on pancreas and bone via sex steroids released by the gonads; filled red arrow indicates corticotrope action on the adrenal cortex (via paracrine FSH intra-pituitary action); dashed red arrow indicates the effect of elevated corticosterone levels on the liver). Error bars represent the standard error of the mean. ** = P < 0.01. For statistical details, including individual P-values, see Supplementary Data 1. Source data are provided as a Source Data file.