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. Author manuscript; available in PMC: 2023 Feb 26.
Published in final edited form as: J Invest Dermatol. 2021 Aug 27;142(3 Pt A):583–593.e5. doi: 10.1016/j.jid.2021.08.397

Figure 4. Molecular analysis of OVOL1 targets in the epidermis.

Figure 4.

(a, b) RT-qPCR analysis at (a) 6 and (b) 24 h after IMQ treatment. n = 5 for Ovol1−/− and 4 for control littermates in a; note that two pairs of these mice were also used for RNA-seq analysis. n = 5 pairs in b. (c) ChIP-qPCR for the indicated genes in epidermal cells isolated from 2–6 h post-IMQ–treated adult skin. IgG control values were normalized to 1 for all. Results are summarized from one to four independent experiments. *** P < 0.005; * P < 0.05. P-values were calculated using a two-tailed Student’s paired t-test. Error bars represent mean ± SEM. ChIP-qPCR, chromatin immunoprecipitation coupled with real-time qPCR; h, hour; IMQ, imiquimod; RNA-seq, RNA sequencing.