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. 2023 Feb 10;14:1062656. doi: 10.3389/fimmu.2023.1062656

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Copyright © 2023 Xi, Xiao, An, Liu, Liu, Hao, Wang, Song, Yu and Gu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunization of bamboo sharks with OGT. (A) Results of ncOGT protein isolation and purification. MW, protein molecular markers; Lane 1: bacterial lysate supernatant by pET28a-ncOGT; Lane 2: penetrating liquid; Lane 3: 20 mM imidazole eluent; Lane 5-13: the purified OGT protein. SDS-PAGE gel was stained using Coomassie Brilliant Blue. (B) The injection and bleeding sites. (C) The immunization schedules.