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. 2023 Feb 10;14:1062656. doi: 10.3389/fimmu.2023.1062656

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Copyright © 2023 Xi, Xiao, An, Liu, Liu, Hao, Wang, Song, Yu and Gu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cytosolic and cytoplasmic localization of OGT. (A) Binding of anti-OGT/O-linked N-acetylglucosaminyltransferase (ab96718); (B) 2D9; (C) 3F7 in wild-type H1299 cells, respectively. Immunofluorescence images representing OGT (Red) and DAPI (Blue) were overlaid (Merge) to show localization within the nucleus. (D–F). Commercial anti-OGT antibody (D), 2D9 (E), and 3F7 (F) were each co-localized with p3×flag-ncOGT transfected H1299 cells. Immunofluorescence images of OGT represented in green, the three antibodies represented in Red and DAPI Blue were overlaid (Merge) to show co-localization.