FIG. 2.
Expression of late-sporulation genes in pbpF and pbpG mutant strains. Cultures of wild-type (□), pbpF (◊), and pbpG (○) single-mutant, and pbpG pbpF double-mutant (▴) strains carrying fusions of lacZ to late sporulation genes were sampled following initiation of sporulation in 2× SG medium at 37°C. β-Galactosidase expression from sspB-lacZ under regulatory control of ςG (A) or from cotD-lacZ (B) and gerE-lacZ (C) under regulatory control of ςK was assayed using o-nitrophenyl-β-d-galactopyranoside as previously described (31). The cause of cotD overexpression in the pbpF mutants is unknown.