TABLE 1.
B. subtilis strains used
| Strain | Genotypea | Transformation
|
Source or reference | |
|---|---|---|---|---|
| Donor(s) | Recipient | |||
| IA626 | hisA82::Tn917 | Bacillus Genetic Stock Center | ||
| AD51 | gerE-lacZ | 11 | ||
| AD52 | cotD-lacZ | 58 | ||
| AD799 | sspB-lacZ | 26 | ||
| DPVB29 | Double ΔpbpD::Cm | pDPC271 | PS832 | This work |
| DPVB30 | ΔpbpD (Spo−) | This work | ||
| DPVB40 | ΔpbpD hisA82::Tn917 (Spo+) | 1A626 | DPVB30 | This work |
| DPVB42 | ΔpbpD (Spo+) | DPVB30 | DPVB40 | This work |
| DPVB45 | ΔpbpG::Kn | pDPV35 | PS832 | This work |
| DPVB46 | ΔpbpD ΔpbpF::Ermr | PS1869 | DPVB42 | This work |
| DPVB49 | ΔpbpD ΔpbpG::KnΔpbpF::Ermr | DPVB45 | DPVB46 | This work |
| DPVB56 | ΔpbpG::KnΔpbpF::Ermr | DPVB45 | PS1869 | This work |
| DPVB57 | ΔpbpD ΔpbpG::Kn | DPVB45 | DPVB42 | This work |
| DPVB61 | ΔponA::Sp ΔpbpG::Kn | PS2062 | DPVB45 | This work |
| DPVB62 | ΔponA::Sp ΔpbpD ΔpbpG::Kn | PS2062 | DPVB57 | This work |
| DPVB63 | ΔponA::Sp ΔpbpG::Kn ΔpbpF::Ermr | PS2062 | DPVB56 | This work |
| DPVB68 | ΔponA::Sp ΔpbpD | PS2062 | DPVB42 | This work |
| DPVB69 | ΔponA::Sp ΔpbpD ΔpbpF::Ermr | PS2062 | DPVB46 | This work |
| DPVB84 | sspB-lacZ | AD799 | PS832 | This work |
| DPVB85 | sspB-lacZ ΔpbpF::Ermr | AD799 | PS1869 | This work |
| DPVB86 | sspB-lacZ ΔpbpG::Kn | AD799 | DPVB45 | This work |
| DPVB89 | sspB-lacZ ΔpbpG::Kn ΔpbpF::Ermr | DPVB84 | DPVB56 | This work |
| DPVB90 | gerE-lacZ | AD51 | PS832 | This work |
| DPVB91 | gerE-lacZ ΔpbpF::Ermr | AD51 | PS1869 | This work |
| DPVB92 | gerE-lacZ ΔpbpG::Kn | AD51 | DPVB45 | This work |
| DPVB93 | gerE-lacZ ΔpbpG::Kn ΔpbpF::Ermr | AD51 | DPVB56 | This work |
| DPVB94 | cotD-lacZ | AD52 | PS832 | This work |
| DPVB95 | cotD-lacZ ΔpbpF::Ermr | AD52 | PS1869 | This work |
| DPVB96 | cotD-lacZ ΔpbpG::Kn | AD52 | DPVB45 | This work |
| DPVB97 | cotD-lacZ ΔpbpG::Kn ΔpbpF::Ermr | AD52 | DPVB56 | This work |
| DPVB141 | ΔpbpG::Kn cwlD::Cm | PS2307 | DPVB45 | This work |
| DPVB142 | ΔpbpF::ErmrcwlD::Cm | PS2307 | PS1869 | This work |
| DPVB143 | ΔpbpG::Kn ΔpbpF::Erm cwlD::Cm | PS2307 | DPVB56 | This work |
| DPVB157 | ΔpbpF::Erm sleB::Sp cwlJ::Tetr | FB111, FB112 | PS1869 | This work |
| DPVB158 | ΔpbpG::Kn sleB::Sp cwlJ::Tetr | FB111, FB112 | DPVB45 | This work |
| DPVB159 | ΔpbpG::Kn ΔpbpF::ErmrsleB::Sp cwlJ::Tetr | FB111, FB112 | DPVB56 | This work |
| FB111 | cwlJ::Tetr | 33 | ||
| FB112 | sleB::Sp | 33 | ||
| FB113 | cwlJ::TetrsleB::Sp | 33 | ||
| PS832 | Prototrophic revertant of strain 168 | Laboratory stock | ||
| PS1869 | ΔpbpF::Ermr | pDPC89 | PS832 | 42 |
| PS2062 | ΔponA::Sp | pDPC197 | PS832 | 41 |
| PS2251 | ΔponA::Sp ΔpbpF::Ermr | PS2062 | PS1869 | 44 |
| PS2307 | cwlD::Cm | ADD1 | PS832 | 45 |
a
Abbreviations: Ermr resistance to erythromycin and lincomycin; Sp, resistance to spectinomycin; Cm, resistance to chloramphenicol; Kn, resistance to kanamycin; Tetr, resistance to tetracycline.