| Antibodies |
| BUV395 anti-CD45 |
BD Biosciences |
Cat# 564279 |
| BV421 anti-ICAM-1 |
BioLegend |
Cat# 116141 |
| BV480 anti-MHC II |
BD Biosciences |
Cat# 566086 |
| BV650 anti-CD11b |
BioLegend |
Cat# 101259 |
| PE-Cy7 anti-Ly6G |
BioLegend |
Cat# 127618 |
| PE anti-C3 |
Novus Biologicals |
Cat# NB200-540PE |
| AF647 anti-C3 |
Novus Biologicals |
Cat# NB200-540AF647 |
| APC-R700 anti-CD11c |
BD Biosciences |
Cat# 565872 |
| APC-Cy7 anti-Ly6C |
BioLegend |
Cat# 128026 |
| Purified anti-CD16/CD32 |
Bio X Cell |
Cat# BE0307 |
| FITC anti-CD45 |
BioLegend |
Cat# 103108 |
| PE anti-Ly6G |
BioLegend |
Cat# 127608 |
| APC-Cy7 anti-CD11b |
BioLegend |
Cat# 101216 |
| APC anti-TNFR1 |
BioLegend |
Cat# 113006 |
| Purified anti-gp75 |
Bio X Cell |
Cat# BE0151 |
| FITC anti-DNA/RNA damage |
Abcam |
Cat# ab183393 |
| AF488 anti-FITC |
ThermoFisher Scientific |
Cat# A11096 |
| APC-Fire 750 anti-CD11b |
BioLegend |
Cat# 101262 |
| FITC anti-ICAM-1 |
BioLegend |
Cat# 353108 |
| BV711 anti-CD16 |
BioLegend |
Cat# 302044 |
| BV786 anti-CD32 |
BD Biosciences |
Cat# 564840 |
| AF647 anti-CD66b |
BioLegend |
Cat# 305110 |
| BV510 anti-CD63 |
BD Biosciences |
Cat# 740182 |
| PE anti-C5AR1 |
BioLegend |
Cat# 344304 |
| AF647 anti-CD177 |
BD Biosciences |
Cat# 566599 |
| FITC anti-SIRPa |
BioLegend |
Cat# 144006 |
| BV480 anti-Siglec F |
BD Biosciences |
Cat# 746668 |
| PE-Cy7 anti-CD101 |
ThermoFisher Scientific |
Cat# 25-1011-82 |
| APC-Cy7 anti-CD14 |
BioLegend |
Cat# 123316 |
| BV711 anti-PD-L1 |
BioLegend |
Cat# 124319 |
| APC anti-XCR1 |
BioLegend |
Cat# 148206 |
| APC-Cy7 anti-F4/80 |
BioLegend |
Cat# 123118 |
| FITC anti-CD206 |
BioLegend |
Cat# 141704 |
| PerCP-eF710 anti-CD80 |
ThermoFisher Scientific |
Cat# 46-0801-82 |
| BUV737 anti-CD86 |
BD Biosciences |
Cat# 741737 |
| BV421 anti-PD-L1 |
BioLegend |
Cat# 124315 |
| BUV737 anti-Ly6G |
BD Biosciences |
Cat# 741813 |
| BV785 anti-Ly6C |
BioLegend |
Cat# 128041 |
| BV421 anti-TCRb |
BioLegend |
Cat# 109230 |
| PE-Cy7 anti-NK1.1 |
BioLegend |
Cat# 108714 |
| PE anti-KLRG1 |
BioLegend |
Cat# 138408 |
| BV510 anti-CD8a |
BioLegend |
Cat# 100752 |
| BV711 anti-CD4 |
BioLegend |
Cat# 100557 |
| B220 anti-BV711 |
BioLegend |
Cat# 103255 |
| AF488 anti-Foxp3 |
BD Biosciences |
Cat# 560403 |
| BV650 anti-CD25 |
BioLegend |
Cat# 102038 |
| PerCP-Cy5.5 anti-CD62L |
BioLegend |
Cat# 104432 |
| APC-eF780 anti-CD44 |
ThermoFisher Scientific |
Cat# 47-0441-82 |
| BV785 anti-PD-1 |
BioLegend |
Cat# 135225 |
| APC anti-CD69 |
BD Biosciences |
Cat# 560689 |
| AF700 anti-Ki67 |
ThermoFisher Scientific |
Cat# 56-5698-82 |
| eF450 anti-CD34 |
ThermoFisher Scientific |
Cat# 48-0341-82 |
| AF700 anti-IL-7Ra |
ThermoFisher Scientific |
Cat# 56-1271-82 |
| BV711 anti-Sca-1 |
BioLegend |
Cat# 108131 |
| FITC anti-cKit |
BD Biosciences |
Cat# 553354 |
| PE anti-CD16/32 |
BioLegend |
Cat# 156606 |
| PE-Cy7 anti-CD3e |
ThermoFisher Scientific |
Cat# 25-0032-82 |
| PE-Cy7 anti-CD4 |
ThermoFisher Scientific |
Cat# 25-0041-82 |
| PE-Cy7 anti-CD8a |
ThermoFisher Scientific |
Cat# 25-0081-82 |
| PE-Cy7 anti-CD11b |
ThermoFisher Scientific |
Cat# 25-0112-82 |
| PE-Cy7 anti-B220 |
ThermoFisher Scientific |
Cat# 25-0452-82 |
| APC anti-Flt3 |
BioLegend |
Cat# 135310 |
| Pacific Blue anti-CD106 |
BioLegend |
Cat# 105722 |
| PE-Cy5 anti-Ly6G |
ThermoFisher Scientific |
Cat# 15-9668-82 |
| PerCP-Cy5.5 anti-CD81 |
BioLegend |
Cat# 104911 |
| PE-Cy7 anti-NK1.1 |
ThermoFisher Scientific |
Cat# 25-5941-82 |
| PE-Cy7 anti-Sca-1 |
BioLegend |
Cat# 122514 |
| AF700 anti-CD34 |
ThermoFisher Scientific |
Cat# 56-0341-82 |
| AF647 anti-gp75 |
Novus Biologicals |
Cat# NBP2-34720AF647 |
| PE-Cy7 anti-PD-L1 |
BioLegend |
Cat# 124314 |
| PE anti-H-2Kb |
BioLegend |
Cat# 116508 |
| APC anti-H-2Db |
BioLegend |
Cat# 111514 |
| FITC anti-MPO |
Abcam |
Cat# ab90812 |
| Purified anti-citrullinated H3 |
Abcam |
Cat# ab5103 |
| PE Donkey anti-rabbit IgG |
BioLegend |
Cat# 406421 |
| Biotin anti-PE |
BioLegend |
Cat# 408104 |
| Purified anti-CD40 |
Bio X Cell |
Cat# BE0016-2 |
| Purified anti-CD44 |
RayBiotech |
Cat# DS-MB-00666 |
| Purified anti-MHC Class I |
Bio X Cell |
Cat# BE0180 |
| Purified anti-human MHC Class I |
Bio X Cell |
Cat# BE0079 |
| Purified mouse IgG2a isotype control |
Bio X Cell |
Cat# BE0085 |
| Purified anti-Ly6G |
Bio X Cell |
Cat# BE0075-1 |
| Purified rat IgG2a isotype control |
Bio X Cell |
Cat# BE0089 |
| Purified anti-TNFR1 |
BioLegend |
Cat# 112906 |
| Purified anti-TNFR2 |
BioLegend |
Cat# 113307 |
| Purified Armenian Hamster IgG |
Bio X Cell |
Cat# BE0091 |
| Purified anti-Factor B, produced from ATCC hybridoma |
ATCC |
Cat# PTA-6230 |
| Purified mouse IgG1 isotype control |
Bio X Cell |
Cat# BE0083 |
| Purified anti-properdin |
Produced as in Miwa et al., 2013
|
N/A |
| Purified anti-C5 |
Produced as in Miwa et al., 2013
|
N/A |
| Purified anti-C5AR1 |
BioLegend |
Cat# 135816 |
| Purified rat IgG2b isotype control |
Bio X Cell |
Cat# BE0090 |
| Purified anti-CD40 |
Bio X Cell |
Cat# BE0189 |
| Purified anti-EGFR |
Bio X Cell |
Cat# SIM0002 |
| Purified anti-GFP |
Bio X Cell |
Cat# RT0265 |
| Biological samples |
| De-identified human whole blood |
Stanford Blood Center |
N/A |
| Chemicals, peptides, and recombinant proteins |
| Recombinant mouse TNF |
BioLegend |
Cat# 575208 |
| Recombinant mouse GM-CSF |
BioLegend |
Cat# 576302 |
| Recombinant mouse IFNγ |
BioLegend |
Cat# 575304 |
| Recombinant mouse IL-1β |
BioLegend |
Cat# 575102 |
| Recombinant mouse IL-17A |
BioLegend |
Cat# 576002 |
| Recombinant mouse C5a |
R&D Systems |
Cat# 2150-C5-025/CF |
| Recombinant human TNF |
BioLegend |
Cat# 570104 |
| Recombinant human C5a |
R&D Systems |
Cat# 2037-C5-025/CF |
| Cobra venom factor |
Millipore Sigma |
Cat# 233552-1MG |
| SC57461A |
Cayman Chemical |
Cat# 10108 |
| CP-105696 |
Millipore Sigma |
Cat# PZ0363-25MG |
| Reduced L-glutathione |
Millipore Sigma |
Cat# G4251-25G |
| Catalase |
Millipore Sigma |
Cat# C40-500MG |
| Topiroxostat |
MedChem Express |
Cat# HY-14874 |
| Luminol sodium salt |
Sigma Aldrich |
Cat# A4685 |
| DiD’ solid |
ThermoFisher Scientific |
Cat# D7757 |
| Critical commercial assays |
| In Situ Cell Death Detection Kit, TMR Red |
Millipore Sigma |
Cat# 12156792910 |
| OxyBURST Green H2HFF BSA |
ThermoFisher Scientific |
Cat# O13291
|
| Leukotriene B4 ELISA Kit |
Cayman Chemical |
Cat# 520111 |
| GSH/GSSG Ratio Detection Kit II |
Abcam |
Cat# ab205811 |
| Xanthine oxidase activity kit |
Abcam |
Cat# ab102522 |
| DELFIA EuTDA cytotoxicity reagents |
PerkinElmer |
Cat# AD0116 |
| Experimental models: Cell lines |
| Mouse: B16F10 |
ATCC |
Cat# CRL-6475 |
| Mouse: LL/2 |
ATCC |
Cat# CRL-1642 |
| Mouse: 4T1 |
ATCC |
Cat# CRL-2539 |
| Human: A549 |
ATCC |
Cat# CRM-CCL-185 |
| Human: MDA-MD-231 |
ATCC |
Cat# CRM-HTB-26 |
| Experimental models: Organisms/strains |
| Mouse: C57BL6/J |
Jackson Laboratory |
Cat# 000664 |
| Mouse: BALB/cJ |
Jackson Laboratory |
Cat# 000651 |
| Mouse: MMTV-PyMT: FVB/N-Tg(MMTV-PyVT)634Mul/J |
Jackson Laboratory |
Cat# 002374 |
| Mouse: Rag2−/−: B6(Cg)-Rag2tm1.1Cgn/J |
Jackson Laboratory |
Cat# 008449 |
| Mouse: TNFR KO: B6.129S-Tnfrsf1atm1ImxTnfrsf1btm1Imx/J |
Jackson Laboratory |
Cat# 003243 |
| Mouse: C3−/−: B6.129S4-C3tm1Crr/J |
Jackson Laboratory |
Cat# 029661 |
| Mouse: Ncf1−/−: B6N.129S2-Ncf1tm1Shl/J |
Jackson Laboratory |
Cat# 027331 |
| Mouse: Rag2−/− Il2rg−/−: B6(Cg)-Rag2tm1.1Cgn/J crossed with B6.129S4-Il2rgtm1Wjl/J |
Jackson Laboratory |
Cat# 008449 × Cat# 003174 |
| Mouse: Cfp−/− |
Kimura et al., 2010
|
N/A |
| Mouse: C6−/− |
Ueda et al., 2019
|
N/A |
| Mouse: Fcer1g−/−: B6.129P2-Fcer1gtm1Rav N12 |
Taconic |
Cat# 583 |
| Oligonucleotides |
| See Table S1 for oligonucleotide information |
|
|
| Recombinant DNA |
| pSpCas9(BB)-2A-GFP (PX458) |
Ran et al., 2013
|
Addgene # 48138; RRID:Addgene_48138 |
| pLVX-EF1a-IRES-Puro |
Clontech |
Cat# 631988 |
| Software and algorithms |
| BD FACSDiva (v8) |
BD Biosciences |
https://www.bdbiosciences.com/en-us/products/software/instrument-software/bd-facsdiva-software
|
| Zen black edition |
Zeiss |
https://www.zeiss.com/microscopy/us/products/microscope-software/zen.html
|
| Zen blue edition |
Zeiss |
https://www.zeiss.com/microscopy/us/products/microscope-software/zen.html
|
| BZ-X800 Viewer |
Keyence |
https://www.keyence.com/
|
| BZ-X800 Analyzer |
Keyence |
https://www.keyence.com/
|
| WorkOut 2.5 |
PerkinElmer |
https://www.perkinelmer.com/
|
| FlowJo (v10) |
BD Biosciences |
https://www.flowjo.com/solutions/flowjo/downloads
|
| SpectroFlo V2.2.0.3 |
Cytek Biosciences |
https://cytekbio.com/blogs/resources/spectroflo-v2-2-0-3-release-notes
|
| Living Image |
Caliper Life Sciences |
https://www.perkinelmer.com/Product/li-software-for-lumina-1-seat-add-on-128110
|
| ImageJ |
Fiji |
https://imagej.net/software/fiji/downloads
|
| Photoshop CS6 |
Adobe |
https://www.adobe.com/products/photoshop.html
|
| Excel |
Microsoft |
https://www.microsoft.com/en-us/microsoft-365/excel
|
| GraphPad Prism (v9) |
GraphPad |
https://www.graphpad.com/scientific-software/prism/
|
