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Type and Level of Experimental Parameters and the Critical Quality Attributes Used in Central Composite Design
| Independent Variables | Level | ||
|---|---|---|---|
| Low | Mid | High | |
| BM concentration, % (w/v), (X1) | 3 | 4.5 | 6 |
| Total Surfactant concentration, % (w/v), (X2) | 5 | 7.5 | 10 |
| Sonication time, min (X3) | 2 | 4 | 6 |
| Fixed experimental factors | |||
| Sonication amplitude, % | 35% | ||
| Homogenization speed, rpm | 11,000 | ||
| Homogenization time, min | 1 | ||
| Dependent variables | Constraints | ||
| PS, nm (Y1) | Minimize | ||
| PDI, (Y2) | Minimize | ||
| ZP, mV (Y3) | Maximize | ||
