Table 5.

Proposed Experiments by Central Composite Design and Their Responses for 20 Formulations of Imiquimod-Loaded Nanostructured Lipid Carriers

Run	Experimental Factors			Dependent Variables
Formulation	X1	X2	X3	Y1	Y2	Y3
CCD1	6	10	2	99.5	0.232	−35.2
CCD2	4.5	7.5	4	92.7	0.243	−35.3
CCD3	4.5	7.5	7.3635	82.8	0.264	−36.6
CCD4	4.5	7.5	0.63641	90.1	0.421	−36.9
CCD5	4.5	3.2955	4	138	0.389	−41.3
CCD6	3	5	2	93.8	0.415	−37.9
CCD7	3	10	6	59.8	0.261	−26.0
CCD8	6	10	6	93	0.225	−29.4
CCD9	3	5	6	94.4	0.301	−35.5
CCD10	4.5	7.5	4	85.3	0.238	−32.8
CCD11	1.9773	7.5	4	59.5	0.23	−27.2
CCD12	4.5	11.7044	4	75.2	0.243	−28.8
CCD13	4.5	7.5	4	86.9	0.222	−35.5
CCD14	7.0226	7.5	4	116.4	0.258	−35.4
CCD15	6	5	2	144.8	0.431	−41.1
CCD16	4.5	7.5	4	85.4	0.243	−36.4
CCD17	4.5	7.5	4	86.3	0.242	−35.6
CCD18	6	5	6	127.5	0.261	−40.6
CCD19	3	10	2	63.3	0.246	−30.0
CCD20	4.5	7.5	4	92.6	0.263	−36.0

Abbreviations: X₁, Binary mixture concentration (% w/v); X₂, Total surfactant concentration (% w/v); X₃, Sonication time; Y₁, PS (nm); Y₂, Polydispersity Index; Y₃, Zeta-potential (mV).