Figure 1. Plant Diagram of the drainage conduit and the sponge frames allocation.

Schematic drawing (not in scale) and real picture of the wastewater drainage conduit where the sponge Hymeniacidon perlevis lives (modified from Longo et al., 2022) with polyvinyl chloride (PVC) frames utilized for sponge volume measurement. In the red boxes picts of H. perlevis within a PVC frame and a specimen collected for analysis. The blue arrow indicates the wastewater flow direction.