TABLE 1.
Escherichia coli K-12 and intR1 derivatives
| Designation | Relevant genotypea | Origin |
|---|---|---|
| BR3674 | recA::Tn10 | D. K. Chattoraj |
| CC3775 | recD1901::Tn10 | J. Sawitzke |
| EC1005 | metB nalA relA1 spoT1 Ir F− | 14 |
| EC1005 tus::Kanr | EC1005 × P1 (PK2517) | This work |
| GY6781 | Δ(pro-lac) mal::Tn9 lexA1(Ind−) rpsL sfiA::lacZ | R. Devoret |
| JAS34 | recF332::Tn3 | J. A. Sawitzke |
| JAS73 | recJ2001::Kanr | J. A. Sawitzke |
| KHG962 | tus::Tn3 (W3110 background) | T. Horiuchi |
| MG1655 | λ− F− | 19 |
| MG1655 tus::Kanr | MG1655 × P1 (PK2517) | This work |
| N2057 | ruvA60::Tn10 | R. G. Lloyd |
| N2101 | recB268::Tn10 | 31 |
| N3793 | recG263::Kanr | R. G. Lloyd |
| N8954 | recA::Cmr (JM103 background) | J. L. Rosner |
| PK1012 | P2sig5; prophage inserted at 47 min; dnaA(Ts) | 15 |
| PK2517 | tus::Kanr | P. Kuempel |
| SK129 | recB270 recC271 (AB1157 background) | 26 |
| TH765 | srl300::Tn10 recA200(Ts) λ−argA21 spoT thi-1 deoB13 | T. Hill, 32 |
| intR1 strains | ||
| EC::71CW | EC1005 ΔoriC::pOU71; rep-Td; Ampr; initiating clockwise | 4 |
| EC::71CC | EC1005 ΔoriC::pOU71; rep-Td; Ampr; initiating counterclockwise | 4 |
| EC::71CW tus::Kanr | EC::71CW-L × P1 (PK2517) | 9 |
| EC::71CC tus::Kanr | EC::71CC-L × P1 (PK2517) | 9 |
| LK211 | ΔoriC::pKN1562; Kanr; initiating clockwise | 23 |
| LK348 | ΔoriC::pKN1562; Kanr; initiating counterclockwise | 23 |
| MG::71CW-L | MG1655 ΔoriC::pOU71; rep-Td; Ampr; initiating clockwise | 34 |
| MG::71CC-L | MG1655 ΔoriC::pOU71; rep-Td; Ampr; initiating counterclockwise | 34 |
| MG::71CW-L tus::Kanr | MG::71CW-L × P1 (PK2517) | This work |
| MG::71CC-L tus::Kanr | MG::71CC-L × P1 (PK2517) | This work |
| MG::71CC-L tus::Kanr-pBADtus | MG::71CC-L tus::Kanr transformed with pBADtus (Cmr) (pBRI) | B. Peter, this work |
| MG::71CC-L tus::KanrrecA::Tn10 | MG::71CC-L tus::Kanr × P1 (BR3674) | This work |
| MG::71CC-L tus::KanrrecA::Tn10-pBADtus | MG::71CC-L tus::KanrrecA::Tn10 transformed with pBADtus (Cmr) | B. Peter, this work |
| TH765::CC | TH765 × P1 (LK348) | This work |
| TH765::CC tus::Tn3 | TH765::CC × P1 (KHG962) | This work |
a
Symbols according to Berlyn et al. (2). Amp, ampicillin; Cm, chloramphenicol; Kan, kanamycin; IN, chromosomal inversion; rep-Td, replication temperature dependent. Tn3 confers ampicillin resistance, and Tn10 confers tetracyclin resistance.