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An example of plaque assay outcome obtained in a 12-well plate culture
| NO. Of plaques | 106 dilution |
107 dilution |
108 dilution |
Negative control |
|---|---|---|---|---|
| Column 1 | Column 2 | Column 3 | Column 4 | |
| Row 1 | 18 | 2 | 0 | 0 |
| Row 2 | 25 | 2 | 0 | 0 |
| Row 3 | 29 | 3 | 0 | 0 |
PFU of virus shown above = ((2 + 2+3)/3)×107/0.2 = 1.33 × 108 PFU/mL (Column 2).
Or = ((18 + 25+29)/3)×106/0.2 = 1.2 × 108 PFU/mL (Column 1).
