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Complete RPMI medium
| Reagent | Final concentration | Amount |
|---|---|---|
| RPMI | N/A | 500 mL |
| FBS | 10% | 60 mL |
| Penicillin/streptomycin | 100 Units/mL/100 μg/mL | 6 mL |
| Na pyruvate | 0.67 mM | 4 mL |
| NEAA | 1× | 6 mL |
| Na-bicarbonate | 0.15% | 12 mL |
| L-glutamine | 4 mM | 12 mL |
| Total | N/A | 600 mL |
Storage: 4°C, 2 weeks.
