pVR21 Digestion mixture
| Reagent | Final concentration | Amount |
|---|---|---|
| Buffer3 | N/A | 6.5 μL |
| 100× BSA | 1.1× | 0.7 μL |
| Not I | 8.57 Units/mL | 2.0 μL |
| H2O | N/A | 5.8 μL |
| pDNA | N/A | 50.0 μL |
| Total | N/A | 65.0 μL |
Storage: prepare right before usage.
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