Transcription mixture
| Reagent | Final concentration | Amount |
|---|---|---|
| pDNA | N/A | 7.5 μL |
| 10× Buffer | 1× | 3.0 μL |
| 2× NTP/CAP | 8.57 Units/mL | 15.0 μL |
| Enzyme | N/A | 3.0 μL |
| GTP | N/A | 1.5 μL |
| Total | N/A | 30.0 μL |
Storage: prepare right before usage.
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