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. 2023 Feb 27;4(2):102171. doi: 10.1016/j.xpro.2023.102171

Mouse tissue digestion buffer

Reagent Final concentration Amount
type III Collagenase 3 mg/mL 1.5 g
DNase I 5 μg/mL 2.5 mg
1× PBS N/A 500 mL
Total N/A 500 mL

Storage: aliquot into 10 mL/tube, −20°C, 6 months.