REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Brilliant Violet (BV) 510 anti-mouse CD45	BioLegend	103138
APC/Cyanine 7 anti-mouse CD3e	BioLegend	100330
PE/Cyanine 5 anti-mouse CD4	BioLegend	100410
PE/Cyanine 7 anti-mouse CD8	BioLegend	100722
APC anti-mouse IFN-γ	BioLegend	505810
FITC anti-mouse TNF	BioLegend	506304
PE anti-mouse IL2	BioLegend	503808
FcγR blocker	BD	553141
FITC goat anti-mouse IgG	Abcam	Ab6785
Bacterial and virus strains
MERS-CoV (EMC2012 strain or equivalent)	Erasmus Medical Center, Rotterdam, Netherlands	N/A
Chemicals, peptides, and recombinant proteins
DMEM powder	Gibco	12100-046
DMEM solution	Gibco	11960-044
MEM	Gibco	M200500
RPMI	Gibco	11875119
FBS	Atlanta Biologicals	s11150
Penicillin/streptomycin	Gibco	15140-122
Na-pyruvate	Gibco	11360-070
NEAA	Gibco	11140-050
Na-bicarbonate	Gibco	25080-094
L-glutamine	Gibco	25030-081
ACK buffer	Gibco	A10492-01
1× PBS	Gibco	10010-023
1× PBS (Mg++ and Ca++)	Gibco	14040-133
Sucrose	Invitrogen	15503022
0.25% Trypsin-EDTA	Gibco	25200-056
Type 3 collagenase	Worthington Biochem	LS004180
DNase I	Worthington Biochem	LS006330
Formaldehyde solution	Sigma	47608-1L-F
Brefeldin A	Sigma	B6542
DMSO	Sigma	67-68-5
Agarose powder	Roche	11685678001
Acetone	Sigma	270725
Methanol	Sigma	34860
Crystal violet powder	Fisher	c581
Ketamine	Vetaket	NDV-59399-114-10
Xylazine	Anased	NDC-59399-111-50
Not I enzyme	Thermo Fisher Scientific	ER0591
T7 Transcription kit	Ambion	AM1334
Virex II 256	Virex	N/A
Micro-Chem	NCL	1839-95-2296
MERS-CoV-specific peptides	Bio-Synthesis	N/A
Experimental models: Organisms/strains
hDPP4-KI mice (C57BL/6J background) 8–10 weeks old, male or female	University of Iowa	N/A
Experimental models: Cell lines
Vero81	ATCC	CCL-81
BHK	ATCC	CCL-10
Software and algorithms
FlowJo software	BD	v10.8
Prism	GraphPad	8
Other
Live/dead cell kit	Invitrogen	L34964
Anti-fade buffer	Vector Laboratories	UX-93952-24
CountBright™ Absolute Counting Beads	Invitrogen	C36950
Cytofix™ Fixation Buffer X2	BD	BDB554655
Cytofix/Cytoperm™ Fixation/Permeabilization Solution Kit	BD	BDB554714
pVR-21 and plasmids encoding the 3526 capsid and E glycoproteins	Dr. Ralph Baric, University of North Carolina	N/A