Figure 5.

Effect of persistent IL-1β exposure on the cell morphology of healthy bovine chondrocytes. Bovine healthy chondrocytes were incubated for 6 days with 10 ng/ml or without IL-1β. Data is representative of (A) area, (B) length, (C) width, (D) circularity, (E) aspect ratio, (F) roundness, and (G) solidity values obtained from individually measured chondrocytes with 1181 cells analyzed for the control group and 2917 cells analyzed for the IL-1β incubation group from n = 8 per group isolated from 3 different cows with samples treated in duplicate or triplicate. Box plots: the boxes define the 25th and 75th percentiles, with a central line at the median; error bars define the 10th and 90th percentiles; and dots define the 5th and 95th percentiles. *p<0.05.