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. 2023 Feb 13;14:1065767. doi: 10.3389/fimmu.2023.1065767

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Copyright © 2023 Petrova, Groth, Bitsch, Arkhypov, Simon, Hetjens, Müller, Utikal and Umansky

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Baseline characteristics of PMN- and M-MDSC from responders and non-responders. (A) Results are presented as the frequency of circulating PNM- and M-MDSC among live PBMC from responders (n=12) and non-responders (n=6). Representative histograms for the proliferation of unstimulated (unstim) and stimulated (stim) T cells incubated alone or in the presence of isolated PMN- or M-MDSC from a non-responding (B) and responding patient (C). (D) Immunosuppressive capacity of PMN- and M-MDSC was determined upon the co-culture with activated CD3 T cells labeled with CP-Dye405. Cumulative data for T cell proliferation are shown as the percentage of divided T cells normalized (norm.) to the respective control of stimulated T cells alone (n=2-8).