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. 2023 Feb 13;11:1138702. doi: 10.3389/fbioe.2023.1138702

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Copyright © 2023 Chen, Xu and Champer.

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Genetic Constructs and target sites. The figure shows each element in several genetic constructs described in this study. Besides these, there is a RpL35A rescue-only construct that is similar to the 2-gRNA TADE drive but lacks the gRNAs and has an intact ECFP fluorescent protein gene instead of DsRed. Another construct is similar to nanos-Cas9, but with the nanos promoter and 5′UTR replaced by vasa elements. Lightning bolts indicate gRNA target sites. The 3xP3 Pol II promoter and the SV40 or P10 3′UTRs/terminators are used to drive the expression of DsRed and EGFP markers, while the Pol III promoter U6:3 is used to express gRNAs.