FIG. 6.

Southern blot analyses of the genomic DNAs of the ORF11- and ORF12-inactivated strains and bioassay for the TP productivity of these mutants and their complemented transformants. SacI-HindIII digests of DNAs from ORF11-inactivated strains (A, lanes 2 to 5) and SacI-BstEII digests of DNAs from ORF12-inactivated strains (B, lanes 2 to 5) were, respectively, subjected to Southern blot analyses with a ³²P-labeled 5.9-kb SacI fragment as a probe. SacI-HindIII (A) and SacI-BstEII digests (B) of DNAs from S. griseolosporeus strain MF730-N6 (wild type) are shown in lane 1. Arrows indicate the sizes of the hybridized fragments. The hybridized fragments are schematically shown. Bioassays for the TP production of the wild-type strain (A, a), the ORF11-inactivated (A, b to e) and ORF12-inactivated (B, b to e) strains, the ORF11-inactivated strain harboring pEN-ORF11 (A, f to i), and the ORF12-inactivated strain harboring pEN-ORF12 (B, f to i) are shown.