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Electrophoresis of overproduced and purified cyclases. Molecular mass markers (lane 1); total soluble proteins from the E. coli harboring pQE30 (lane 2), pQE30-ORF11 (lane 3), or pQE30-ORF12 (lane 4); purified, His-tagged ORF11 (lane 5); and purified, His-tagged ORF12 (lane 6) were analyzed by SDS–10% PAGE. Proteins were stained with Coomassie brilliant blue R-250.
