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HPLC analysis of the product generated by recombinant cyclases. An authentic terpentetriene standard (A) and chloroform extracts of the reaction products generated by the purified, His-tagged ORF11 and ORF12 products in the presence of GGDP (B) or the absence of GGDP (C) were analyzed by reversed-phase HPLC. mAbs, milliabsorbance.
