FIG. 7.

Deletion of the chromosomal nusA gene. (A) Plasmid pJML001 carries a metY-nusA-infB operon fragment containing an in-frame deletion of nusA cloned into the temperature-sensitive allelic replacement vector pMAK705. (B) Genetic organization of the chromosomal metY-nusA-infB operon containing the nusA deletion and that of the complementing nusA⁺ plasmid after resolution of cointegrates formed between plasmid pJML001 and the chromosome of the wild-type strain, MW100. P represents the P₋₁ and P₁ promoters, and T represents the infBt₃ terminator.