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. 2023 Feb 13;14:1106075. doi: 10.3389/fphys.2023.1106075

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Copyright © 2023 Rohbeck, Niersmann, Köhrer, Wachtmeister, Roden, Eckel and Romacho.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Workflow and overview of the number of genes in untreated, palmitate (PA) or PA + HK4-treated HepG2 cells. After 7 h treatment with 200 μM PA and 10 μM HK4, RNA has been isolated from five independent HepG2 cell lysates per treatment group and 3′-mRNA sequencing has been performed with Illumina NextSeq550 system. Thick arrows indicate up or downregulated genes in the first treatment group of the comparison. Differential expression (DE) of genes have been filtered by p-value ≤0.05 and an absolute value of fold change (|FC|) ≥ 1.5.