Fig. 5. The implication of Rab5 in the polarization of macrophages.

(A) Human THP-1 macrophages were treated with conditioned medium, exosomes collected from MDA-MB-231 cells, or were cocultured with MDA-MB-231 cells. The M2 (CD206, IL-10, and CD163) and M1 (iNOS, IL-1β, and TNF-α) markers in THP-1 cells treated with conditioned medium (B) or exosomes (C) or cocultured with MDA-MB-231 cells (D) were determined by qRT-PCR. (E) The expression of M1/M2 macrophage markers in THP-1 cells cocultured with MDA-MB-231 cells with Rab5 overexpression. The mock treatment represents THP-1 cells treated with normal medium alone. Data was represented as mean ± SD. *p < 0.05, **p < 0.001, ***p < 0.0001 vs. mock treatment.