Figure 1.

Xist RNA is not robustly localized at the Xi in mouse and human AT2 cells

(A) Representative field images of Xist RNA FISH in female mouse AT2 cells isolated from lung. Scale bar is 10 micron.

(B) Representative field images of XIST RNA FISH in human female AT2 cells. Scale bar is 10 micron.

(C) Cartoon showing each category of Xist RNA localization pattern observed in AT2 cells.

(D and E) Quantification of XIST RNA localization patterns for (D) mouse AT2 cells and (E) human AT2 cells. Number of nuclei counted is above each column. Statistical significance determined using t test with unequal variance comparing AT2 with fibroblasts for each category of Xist localization pattern (types I, II, III, IV).

(F) qRT-PCR for Xist expression in mouse AT2 cells, transformed female MEFs (with multiple X chromosomes), and primary B cells from female mice. Error bars denote standard error of mean.

(G) qRT-PCR for XIST expression in human AT2 cells and primary female fibroblasts (IMR-90). Error bars denote standard error of mean.