Figure 5.

GSK3β inhibition or knockdown decreases apoptosis in MFS VSMCs

(A and B) Cells were treated for 96 h prior to staining for TUNEL (red) and DAPI (blue).

(C) Quantification was performed in a blinded and unbiased way using ImageJ and a macro.

For the drug treatment groups, comparisons were performed with MFS DMSO. 150 μm scale bars throughout. n = 3–4 independent experiments. Data are represented as mean ± SEM. Cells treated under control condition (DMSO) were also used as controls for Figure S4.