Figure 3.
FKN increases de novo oligodendrocyte genesis after demyelinating injury
(A) Adult WT mice were infused with FKN-647 or BSA-647 into the lateral ventricle and euthanized 3 h later.
(B) Representative images from BSA-647 (top) and FKN-647 (bottom)-infused brains imaged using fluorescence from far-red (FL-647) channel.
(C) Ten-week-old PDGFRαCreERT2;RosaYFP STOP/+ were fed cuprizone chow for 6 weeks. Tamoxifen was injected in the last week of cuprizone treatment. VC/FKN was infused into the lateral ventricle after cuprizone cessation for 7 days and mice were returned to normal chow.
(D) Representative orthogonal slices of z-stack images of midline CC immunostained for YFP (green), CC1 (red), and OLIG2 (blue) from VC- (left) and FKN- (right) infused mice (see 3D schematic for orientation). Arrows indicate YFP+CC1+OLIG2+ and arrowheads YFP+CC1-OLIG2+ cells.
(E–J) Quantification of (D) in rostral (E and F), medial (G and H) and caudal (I and J) CC.
(K) Representative images of medial cortical GM immunostained for YFP (green), CC1 (red), and OLIG2 (blue). Arrows indicate YFP+CC1+OLIG2+ and arrowheads YFP+CC1-OLIG2+ cells.
(L and M) Quantification of (K). Scale bars, 50 μm in (B), 5 μm in (D), 25 μm in (K). Error bars represent SEM. Data were analyzed using unpaired t test, ∗p < 0.05, ∗∗p < 0.01, n = 3 mice per group from at least two independent litters.
See also Figure S3.