FIG. 6.

Assessment of transcriptional readthrough in soxA mutant strains using RT-PCR. RT-PCR experiments using primers in soxC (A) or soxF (B) employed total RNA isolated from strains cultured photomixotrophically with malate as carbon source and thiosulfate as electron donor. Lanes 1 to 3 in each panel are the complete RT-PCRs, while lanes 4 to 6 are control experiments in which reverse transcriptase was omitted. The sources of the RNA were R. sulfidophilum strains 3.1 (lanes 1 and 4), soxA::Ω (lanes 2 and 5), and soxA::Gm^r (lanes 3 and 6).