TABLE 1.
Strain | Culture conditions (electron donor, carbon source)a | Assay supplementb | Mean activity [nmol of cytochrome c reduced min−1 (ml of periplasmic extract)−1] |
---|---|---|---|
Parental 3.1 | S2O32−, HCO3− | 12 ± 2 | |
S2O32−, malate | 27 ± 3 | ||
None, malate | 3.5 ± 0.5 | ||
soxA::Gmr | S2O32−, malate | NDc | |
S2O32−, malate | SoxAX | 18 ± 3 | |
soxA::Ω | S2O32−, malate | ND | |
S2O32−, malate | SoxAX | 9 ± 1 |
Cells were harvested at an optical density at 650 nm in the range of 0.8 to 1.3.
Assays were supplemented with 20 μg of cytochrome c551 complex per ml where indicated.
ND, nondetectable.