Figure 2.

Unlike humanized mice without a thymus (humNSG), CCST and BLT humanized models allow for robust T cell reconstitution

(A) Peripheral blood from mice of the three models was harvested and analyzed by flow cytometry for the level of human cell reconstitution (each dot represents a mouse analyzed at a given time point). Extent of the reconstitution of hCD45⁺ (calculated among total CD45⁺ cells [mouse and human]), hCD3⁺ (among hCD45⁺), hCD19⁺ (among hCD45⁺), and hCD14⁺ (among hCD45⁺) was as shown. A non-linear regression curve was calculated for each subpopulation using the growth exponential association model for all populations except hCD19, for which a two-phase exponential association model was applied.

(B) Graphic representation of the relative proportions of human T cells (CD3⁺), B cells (CD19⁺) and monocytic cells (CD14⁺) in BLT and CCST mice at 5, 10, and 15 weeks post humanization, showing an increase in T cell proportion over time for BLT mice.

(C) Dynamic changes in the level of human CD4⁺ and CD8⁺ T cells within the hCD3⁺ population in peripheral blood of CCST and BLT mice. Non-linear regression curves were calculated using a two-phase exponential association model.

(D and E) (D) Proportions of human CD45⁺ and CD3⁺ and (E) levels of CD4⁺ and CD8⁺ T cells in tissues of CCST and BLT mice at 25 weeks post humanization as measured by flow cytometry. The Mann-Whitney test was applied to compare ranks of two groups. ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001, ^∗∗∗∗p < 0.0001.