Figure 5.

CCST mice are as susceptible to HIV infection as BLT mice

(A) CCST and BLT mice were infected with 200,000 TCID₅₀ of NL4.3-ADA-GFP HIV via intraperitoneal injection or vaginal inoculation and monitored for 7 weeks.

(B) Plasma viral load measured in CCST (blue lines) and BLT (red lines) mice following HIV infection. Shown are median values (+95% confidence interval) from 9–19 (depending on the time point) CCST and 15 BLT mice injected intraperitoneally and 7 CCST and 6 BLT mice inoculated vaginally.

(C) Levels of total and integrated HIV DNA in the spleen, lung, and liver, quantified by real-time PCR.

(D) Frequency of virus-expressing (p24⁺) human CD4⁺ T cells (as defined by hCD3⁺hCD8⁻ owing to HIV-mediated CD4 downregulation) in the spleen, lung, and liver as measured by flow cytometry.

wpi, weeks post infection; LOD, limit of detection. The Mann-Whitney test was applied to compare ranks of two groups. ns, not significant; ^∗p < 0.05, ^∗∗p < 0.01.