Skip to main content
. 2023 Feb 2;18(2):597–612. doi: 10.1016/j.stemcr.2023.01.003

Figure 6.

Figure 6

Comparison of general and HIV-specific T cell response in CCST and BLT mice

(A) Splenocytes from infected CCST and BLT mice were kept untreated or stimulated with PMA/ionomycin, pooled Clade B HIV peptides (env, gag, pol, nef) (2 μg/mL), or lymphocytic choreomeningitis virus (LCMV) peptides (GP61-80, GP276-286) (100 ng/mL). LCMV stimulation served as a specificity control for the assay. Frequencies of cytokine-expressing human T cells were measured intracellularly by flow cytometry. Depicted are overall data in different mice (CCST, 10 mice and BLT, 12 mice). Four dots connecting each line represent an individual mouse.

(B) Splenocytes from uninfected (UI), unstimulated CCST and BLT mice were stimulated with PMA/ionomycin and assessed for IFN-γ expression in T cells as described in (A).

(C) Surface expression of activation markers HLA-DR and CD69 on T cells from the spleen of uninfected CCST (blue) and BLT (red) mice was measured by flow cytometry.

In all panels, each dot is one mouse. The Mann-Whitney test was applied to compare ranks of two groups. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001; ns, not significant.