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. 2023 Feb 13;14:1083917. doi: 10.3389/fmicb.2023.1083917

Table 4.

Changes in SCFAs contents produced during fermentation.

SCFAs(mmol/L) Time CON INU LDSPs
Acetic acid 0 2.76 ± 0.10d, A 2.76 ± 0.10d, A 2.76 ± 0.10d, A
6 5.02 ± 0.12a, C 7.18 ± 0.17c, B 20.72 ± 0.05b, A
12 4.57 ± 0.05c, C 11.75 ± 0.11b, B 20.09 ± 0.13c, A
24 4.90 ± 0.23b, C 28.64 ± 0.17a, B 18.70 ± 0.01a, A
Propionic acid 0 0.86 ± 0.03d, A 0.86 ± 0.03d, A 0.86 ± 0.03d, A
6 1.75 ± 0.10c, C 10.16 ± 0.29c, B 14.60 ± 0.09c, A
12 3.06 ± 0.03b, C 11.71 ± 0.40b, B 17.78 ± 0.14b, A
24 4.43 ± 0.02a, C 15.78 ± 0.20a, B 26.31 ± 0.16a, A
n-Butyric acid 0 0.76 ± 0.02c, A 0.76 ± 0.02d, A 0.76 ± 0.02d, A
6 0.47 ± 0.05d, C 1.17 ± 0.13b, B 15.32 ± 0.18c, A
12 1.80 ± 0.15a, C 1.57 ± 0.04a, B 34.44 ± 0.04b, A
24 0.89 ± 0.07b, C 1.12 ± 0.01c, B 45.10 ± 0.29a, A
i-Butyric acid 0 ND ND ND
6 0.06 ± 0.03b, B ND 0.12 ± 0.05b, A
12 0.20 ± 0.11a, A 0.03 ± 0.01, B 0.32 ± 0.01a, A
24 0.19 ± 0.06a, B 0.13 ± 0.03, B 0.40 ± 0.09a, A
n-Valeric acid 0 ND ND ND
6 0.21 ± 0.37c, A ND ND
12 0.76 ± 0.66b, C 0.60 ± 0.21b, B 2.97 ± 0.19a, A
24 1.83 ± 0.15a, B 1.18 ± 0.23a, A 1.91 ± 0.10b, A
i-Valeric acid 0 ND ND ND
6 ND ND ND
12 ND 1.00 ± 0.05a, B 1.42 ± 0.31a, A
24 ND ND ND
Total acid 0 4.39 ± 0.12d, A 4.39 ± 0.12d, A 4.39 ± 0.12d, A
6 7.46 ± 0.13c, C 18.51 ± 0.08c, B 50.64 ± 0.05c, A
12 10.19 ± 0.16b, C 26.63 ± 0.19b, B 76.69 ± 0.09b, A
24 12.05 ± 0.03a, C 46.72 ± 0.21a, B 92.02 ± 0.13a, A

Data are expressed as mean ± SD (n = 5), a–d (lowercase letter) mean significant differences among different times (p < 0.05) in the same group, and A–C (capital letter) represent significant differences among different groups (p < 0.05) at the same time. CON, the blank control (no additional carbon source supplement); INU, the positive control (INU supplement), and LDSPs, the experimental group (LDSPs supplement); ND, not detected.