. 2023 Feb 16;20:153–161. doi: 10.1016/j.ijppaw.2023.02.003

Table 1.

PCR primers and reaction conditions.

Target locus	Primer	Amplicon Length ^α	Sequence	Annealing temperature
COXI	SSP_COX1_F*	217 bp	5′-TTTGATCCTAGTTCTGGTGGTAATCC-3′	65 °C
COXI	SSP_COX1_R*	217 bp	5′-GTAGCAGCAGTAAAATAAGCACGAGA-3′	65 °C
18S rDNA HVR-I	NEW_HVR_I_F	∼434 bp ^β	5′-GCTCATTATAACAGCTATAGACTACACGGTA-3′	65 °C
18S rDNA HVR-I	NEW_HVR_I_R	∼434 bp ^β	5′-CCACAACAATCATTTTATGCACTTGG-3′	65 °C
18S rDNA HVR-IV	NEW_HVR_IV_F	∼255 bp ^β	5′-CGGGCCGGACACTATAAGG-3′	63 °C
18S rDNA HVR-IV	NEW_HVR_IV_R	∼255 bp ^β	5′-ATCTCTAAACAGGAACATAATGATCACTAC-3′	63 °C

^α Excluding PCR primer sequences from the amplicon.

^β Length varies depending on genotype.

^∗ Broadly specific primers for generation of Strongyloides sp. cox1 amplicons as well as those of several strongyles.