Table 1.

Dissociation constants and thermodynamic values determined by ITC analysis for the binding between the boxcar A or the NT0 and quinine in PBS (pH 7.4), 5 mM MgCl₂ at 15 °C, n = 2.

Aptamer	KD (μM)	ΔH(kcal/mol)	−TΔS (kcal/mol)	Binding sites N
A	0.61 ± 0.07	−12.6 ± 0.6	4.4 ± 0.7	1
NT0	0.53 ± 0.09	−11.8 ± 0.9	3.5 ± 1.0	4