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. 2023 Feb 10;70:101692. doi: 10.1016/j.molmet.2023.101692

Figure 2.

Figure 2

Activation of the HNS increases Glp1r expression in Avp MCNs. A, relative mRNA expression of Glp1r was investigated by qRT-PCR in the SON and PVN of 1 day WD and 1 day salt loaded (SL) male rats. B, relative mRNA expression of Creb3l1 and Glp1r was investigated by qRT-PCR in the SON and PVN of female control and lactating rats. C, relative mRNA expression of Glp1r was investigated by qRT-PCR in the SON and PVN of male rats following a single injection of hypertonic saline. D, qRT-PCR analysis of Glp1r mRNA expression in AVP and OXT punches in control and 3-day WD rat PVN. E, fluorescent in situ hybridisation to mark the distribution of Avp (blue), Oxt (red) and Glp1r (white) mRNAs in control and 3-day WD SON MCNs. F, Glp1r RNAscope in situ hybridization in control and 3-day WD SONs. Graphs show gene expression as a function of Glp1r mRNA dots/cytoplasm (Cyto) or nucleus (Nuc) of AVP and OXT neurones in control and WD conditions. Values are means + SEM of n = 4–7 animals per group. ∗p ≤ 0.05, ∗∗p ≤ 0.01, ∗∗∗p ≤ 0.001. Scale bar = 100 μm.