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. 2023 Feb 10;70:101692. doi: 10.1016/j.molmet.2023.101692

Figure 5.

Figure 5

Assessment of Glp1r KD in the SON reveals changes to ingestive behaviour, MCN signalling pathways, and HNS outflows. A, outline of the experimental protocol involving viral vector deliver of specific shRNAs to KD Glp1r expression bilaterally in the SON. The success of gene KD was confirmed by diminished GLP-1R immunostaining in the SON. Food (B) and water intake (C) and body weight (D) were monitored for 8 weeks after viral delivery. E, endpoint measures of AVP and OXT pituitary stores determined by ELISA in control and KD animals. F, immunoblots of GLP-1R, Tubulin, pERK and tERK immunoreactive bands in SON total protein extracts from control and Glp1r KD SONs. G, densitometry analysis of immunoreactive bands from F. H, immunostaining for pERK in the SON of control and Glp1r KD rats. Values are means + SEM of n = 5–8 animals per group. OC, optic chiasm. ∗p ≤ 0.05, ∗∗p ≤ 0.01, ∗∗∗p ≤ 0.001. Scale bars = 50 μm.