Table 1.
Groups and treatment.
| Primary immunization at 4 days old |
Secondary immunization at 14 days old |
Challenge |
|||||
|---|---|---|---|---|---|---|---|
| Group | Vaccine (dosage) | Adjuvant L.lactis | Adjuant dose (CFU) | Vaccine (dosage) | Adjuvant L.lactis | Adjuant dose (CFU) | Virulent strains (dosage) |
| T1 | 1 | NZ3900/pNZ8149-IL-4-IL-2 | 1 × 107 | 2 | NZ3900/pNZ8149-IL-4-IL-2 | 1 × 107 | 1 |
| T2 | 1 | 1 × 108 | 2 | 1 × 108 | 1 | ||
| T3 | 1 | 1 × 109 | 2 | 1 × 109 | 1 | ||
| T4 | 1 | 1 × 1010 | 2 | 1 × 1010 | 1 | ||
| T5 | 1 | 1 × 1011 | 2 | 1 × 1011 | 1 | ||
| T6 | 1 | No-load NZ3900/pNZ8149 | 1 × 1011 | 2 | No-load NZ3900/pNZ8149 | 1 × 1011 | 1 |
| C1 | 0 | PBS | 0 | 0 | PBS | 0 | 0 |
| C2 | 1 | PBS | 0 | 2 | PBS | 0 | 1 |
| C3 | 0 | PBS | 0 | 0 | PBS | 0 | 0 |
Note: The number of oocysts per dosage of vaccine was 1,000 oocysts of E. tenella precocious strain, 400 oocysts of E. acervulina precocious strain, and 100 oocysts of E. maxima Shanxi precocious strain and Shandong precocious strain respectively.
The number of oocysts per dosage of Eimeria virulent strains for challenge was 4.00 × 104 oocysts of E. tenella virulent strain, 20.00 × 104 oocysts of E. acervulina virulent strain, and 15.00 × 104 oocysts of E. maxima Shanxi virulent strain and Shandong virulent strain respectively.