Fig. 1. Chromosomal integration.

Map of the pGP-Tn7-Cm plasmid designed to include a promoter, secretory signal, and CatB antigen. Insertion occurred between the EcoRI and KpnI restriction sites (a). Linear integrated sequences for each YS1646 construct following FLP-FRT recombination to remove the chloramphenicol resistance gene (b). Growth curves of WT and recombinant YS1646 strains measured by absorbance at OD₆₀₀ over 24 h (n = 4 readings per 30 min interval) (c). Confocal microscopy images of uninfected and infected RAW264.7 cells at 63X magnification. DAPI nuclear stain is represented in blue and mCherry expression is shown in red (d). The scale is set to 20 μm.