Table 1.

Granuloma size and egg abnormality.

Vaccine Group	Granuloma size (μm2) ± SEM	Abnormal egg morphology (%) ± SEM
PBS	56536.2 ± 4727.3	10.9 ± 1.7
WT + rCatB	55304.7 ± 4366.9	15.9 ± 1.4
NHC	40136.1 ± 2497.6*	39.6 ± 2.5****
NHC + rCatB	31620.4 ± 1759.2***	51.9 ± 4.8****

Liver egg granuloma size (μm²) and egg morphology (i.e., loss of internal structure, shrinkage, crenellated periphery) were assessed using Zen Blue software. Each independent experiment consisted of 6–10 granulomas per animal per group. Each experiment was performed twice. Data were shown as mean ± SEM.

Statistical significance was determined by one-way ANOVA with Tukey’s multiple comparisons (*P < 0.05, ***P < 0.001, ****P < 0.0001).