Skip to main content
. 2023 Jan 22;14(3):367–378. doi: 10.7150/jca.81027

Figure 3.

Figure 3

Erianin promotes the overall RNA m6A modification of HuRCSCs. (A) qRT‑PCR results indicating that Erianin upregulated METTL3 and downregulated FTO mRNA expression. **p < 0.01 vs. DMSO; *p < 0.05 vs. DMSO; t test; n = 4. (B) Dot blotting results showing that Erianin downregulated the m6A modification of HuRCSCs mRNA. *p < 0.05 vs. DMSO; t test; n = 4. (C) The results of RIP-PCR indicating that Erianin promoted the m6A modification level of the ALOX12 and P53 mRNAs. **p < 0.01 vs. DMSO; *p < 0.05 vs. DMSO; t test; n = 4. (D) Western blotting results showed that Erianin significantly upregulated METTL3, ALOX12, and p53 levels and downregulated the level of FTO protein. **p < 0.01 vs. DMSO; *p < 0.05 vs. DMSO; t test; n = 4. (D) Western blotting results on siAlox12 and siMettl3 transfected group. (E) The results of MTT assay.