TABLE 1.
New mrcA (PBP 1a) mutantsa
| Strain | PBPs Deleted | Parent | Strain | PBPs Deleted | Parent | Strain | PBPs Deleted | Parent | ||
|---|---|---|---|---|---|---|---|---|---|---|
| CS229-1K | 1a 7 | CS9-19 | CS456-1K | 1a 4 5 7 | CS315-1 | CS541-1K | 1a 4 7 H C | CS441-1 | ||
| CS230-1K | 1a H | CS15-3 | CS457-1K | 1a 4 6 7 | CS316-1 | CS542-1K | 1a 4 5 6 H | CS442-3 | ||
| CS231-1K | 1a 6 | CS17-1 | CS458-1K | 1a 4 7 H | CS317-3 | CS543-1K | 1a 5 6 7 H | CS443-3 | ||
| CS232-1K | 1a 5 | CS12-7 | CS459-1K | 1a 4 7 C | CS318-1 | CS544-1K | 1a 4 5 6 C | CS445-1 | ||
| CS233-1K | 1a 4 | CS11-2 | CS460-1K | 1a 4 C H | CS320-1 | CS545-1K | 1a 4 5 6 7 | CS446-1 | ||
| CS234-1K | 1a C | CS14-2 | CS461-1K | 1a 4 5 6 | CS322-1 | CS546-1K | 1a 4 6 7 C | CS447-1 | ||
| CS367-1K | 1a 4 7 | CS203-1B | CS462-1K | 1a 4 6 H | CS323-3 | CS547-1K | 1a 4 5 7 H | CS448-3 | ||
| CS368-1K | 1a 4 H | CS221-3 | CS463-1K | 1a 4 6 C | CS324-1 | CS548-1K | 1a 4 5 7 C | CS449-2 | ||
| CS369-1K | 1a 4 5 | CS219-1 | CS464-1K | 1a 4 5 H | CS326-1 | CS549-1K | 1a 5 6 7 C | CS450-1 | ||
| CS370-1K | 1a 4 6 | CS220-1 | CS465-1K | 1a 4 5 C | CS327-1 | CS550-1K | 1a 5 7 C H | CS452-2 | ||
| CS375-1K | 1a 6 7 | CS205-1 | CS466-1K | 1a 5 6 7 | CS331-1 | CS551-1K | 1a 4 6 C H | CS453-1 | ||
| CS376-1K | 1a 6 H | CS213-1 | CS467-1K | 1a 6 7 H | CS332-3 | CS552-1K | 1a 4 5 C H | CS454-1 | ||
| CS377-1K | 1a 5 7 | CS204-1 | CS468-1K | 1a 6 C H | CS334-1 | CS553-1K | 1a 6 7 C H | CS455-1 | ||
| CS378-1K | 1a 5 H | CS215-3 | CS469-1K | 1a 5 6 H | CS336-3 | CS554-1K | 1a 5 6 C H | CS470-1 | ||
| CS379-1K | 1a 7 H | CS206-3 | CS470-1K | 1a 5 6 C | CS337-1 | CS614-1K | 1a 4 5 6 7 H | CS531-3 | ||
| CS380-1K | 1a 4 C | CS222-1 | CS471-1K | 1a 7 C H | CS342-1 | CS615-1K | 1a 4 5 6 7 C | CS533-1 | ||
| CS381-1K | 1a 5 C | CS216-2 | CS472-1K | 1a 6 7 C | CS343-1 | CS616-1K | 1a 4 5 7 C H | CS534-1 | ||
| CS382-1K | 1a 6 C | CS213-1 | CS473-1K | 1a 5 7 H | CS345-3 | CS617-1K | 1a 4 6 7 C H | CS535-1 | ||
| CS383-1K | 1a 7 C | CS207-2 | CS474-1K | 1a 5 7 C | CS346-1 | CS618-1K | 1a 5 6 7 C H | CS536-1 | ||
| CS384-1K | 1a C H | CS209-1 | CS475-1K | 1a 5 C H | CS349-1 | CS619-1K | 1a 4 5 6 C H | CS539-1 | ||
| CS385-1K | 1a 5 6 | CS211-2 | CS540-1K | 1a 4 6 7 H | CS440-3 | CS703-1K | 1a 4 5 6 7 C H | CS612-1 |
a
The parental strain from which individual and multiple PBP genes were deleted was E. coli CS109 (W1485 rpoS rph). Each mutant (“Strain” columns) was created by P1 transduction of mrcA::res-npt-res from BMCS04-1K (CS109 mrcA::res-npt-res) into the respective parental strain (“Parent” columns) and selection for kanamycin resistance. The parental strains are described by Denome et al (2). PBPs deleted from individual E. coli strains are abbreviated as follows (PBP, followed by gene name): 1a, = PBP 1a, mrcA; 4 = PBP 4, dacB; 5 = PBP 5, dacA; 6 = PBP 6, dacC; 7 = PBP 7, pbpG; C = AmpC, ampC; H = AmpH, ampH.