a, b, 2-DG uptake per milligram iBAT in female (a) or male (b) lab mice and wildlings at either 18 °C or 28 °C. c-h, 2-DG uptake in iWAT (c, d), pgWAT (e, f) or gastrocnemius muscle (g, h) in female (a, c, e, g) or male (b, d, f, h) lab or wildling mice held at 18 °C and 28 °C. N = 8 lab mice and n = 13 wildlings at 18 °C, n = 8 lab mice and n = 10 wildlings at 28 °C (a, c, e, g). N = 8 lab mice and n = 12 wildlings at 18 °C, n = 8 lab mice and n = 10 wildlings at 28 °C (b, d, f, h). Box plots show median (center line), 75th (upper limit of box) and 25th percentile (lower limit of box) and outliers (whiskers) if values do not exceed 1.5-times interquartile range. NS, not significant. Unpaired two-sided Student’s t test (Gaussian model) (a, b, d, e), two-sided Wilcoxon rank sum test (c, f, g, h). (NS, not significant; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, exact p-values are shown in the Source Data).